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Reagent Center

Fast Lysis Method

  • BioFast Soil Genomic DNA Extraction Kit MORE
    Product Details:

    Kit Components

    Cat#

    BSC21S1

    Components

    50Tests

    SP Buffer

    45ml

    Lysis S Buffer

    5ml

    DA Buffer

    12.5ml

    Binding Buffer

    35ml

    Wash Buffer

    30ml(add 45ml absolute ethanol before use)

    Elution Buffer

    15ml

    Grind Tube

    50tubes

    Spin Column

    50tubes

    Handbook

    1copy

    Introduction

        The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

        At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

        Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

    Product Features:
    Storage

    All reagents, when stored properly, are stable for 18 months.

    Apparatus and Materials to Be Supplied by the User

    *  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000g              * Absolute ethanol

    Important notes

    • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
    • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.
    Experimental data:

    Analysis DNA

    • Absorbance analysis

    Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260,OD280 and OD320.

    Expressions:concentration(μg/ml)=50×OD260×dilution fact

    Target: 2.0≥OD260-320/ OD280-320≥1.7

    Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

    • Agarose Gel Analysis

    0.8~1% Agarose gel

    Real-time Taqman PCR detect


    Manual download
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