"

免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金拥有全球最顶尖的原生APP,每天为您提供千场精彩体育赛事,免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金更有真人、彩票、电子老虎机、真人电子竞技游戏等多种娱乐方式选择,免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金让您尽享娱乐、赛事投注等,且无后顾之忧!

"

Reagent Center

Extraction Method

  • BIOZOL Total RNA Extraction Reagent MORE
    Product Details:

     

    Cat#

     

    BSC51S1

     

    BSC51M1

    BIOZOL-total RNA Extraction reagent

    30 ml

    100 ml

    Handbook

    1copy

    1copy

    General information

        BIOZOL Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue or from bacterium. The operation of the kit is very simple and easy. First, add the sample into BIOZOL for complete lyses. Second, add chloroform into the solution and then centrifuge the sample tube. Then the homogenate will separate into form three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

        Since the operation of BIOZOL is very easy, it can realize the extraction for several samples simultaneously. And total RNA isolated by BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

    RNA Extraction Procedure

     


    Product Features:

    Storage and Transportation

    ◆   Recommended to store at 2-8℃ and the stability is one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the effect.

    ◆   It can be shipped at room temperature which does not affect its stability.

    Important Notes

        RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

    ◆    Always wear gloves and respirator in order to prevent RNA degradation.

    ◆    Use sterile, disposable plastic ware and automatic pipettes.

    ◆    All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6-8 hours, while  tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temp in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.

    ◆    Build a separate RNA laboratory with the only instruments if possible.

    Experimental data:

     

    Total RNA pictures above are strictly operated according to Biozol extraction protocol.


    Manual download
用户隐私条款

尊重用户个人隐私是杭州博日用户服务的一项基本政策。杭州博日不会在未经合法用户授权时向第三方公开或透露其注册资料及保存在杭州博日平台中的非公开内容,但以下情况除外: ⑴用户对自身信息保密不当原因,导致用户非公开信息泄露; ⑵由于网络线路、黑客攻击、计算机病毒、政府管制等原因造成的资料泄露免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金、丢失、被盗用或被篡改等; ⑶有关法律要求杭州博日官网服务管理要求提供用户的个人信息免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金; ⑷在紧急情况下为维护用户和公众的生命、财产安全免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金。

免责条款

杭州博日科技不保证本网站上任何内容的准确性、完整性和可靠性以及使用这些内容可能得出的任何结果。?杭州博日科技及相关人不能保证您任何时候均可进入、浏览和使用本网站;杭州博日科技对本网站和其内容的不能使用和使用错误不承担任何责任。?本网站对其他网站的链接本身并不表示杭州博日科技认可或承担其他网站内容或使用上的责任。杭州博日科技以及相关人对于在进入、浏览和使用本网站、从本网站下载任何内容或通过本网站的任何链接进入其他网站而导致病毒或其他破坏性程序对您的电脑系统以及其他任何软件、硬件免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金、IT系统或财产的损害或损失不承担任何责任。?杭州博日科技以及相关人对于第三人采用非法手段进入本网站取得有关密码免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金、资料和内容而引起的任何直接及间接损害或损失不承担任何责任。最终解释权归杭州博日科技所有。

杭州博日保留随时更正免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金、修改、更新本声明的权利免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金。

使用条款

本网站由杭州博日科技有限公司(以下简称“杭州博日”)创设,本网站提供的任何内容(包括但不限于文字、数据、图表、图象、声音或视频等)的版权均属于杭州博日或相关权利人。未经杭州博日或相关权利人事先的书面许可免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金,您不得以任何方式擅自复制、再造、传播、出版、转帖、改编或陈列本网站的内容。任何未经授权使用本网站的行为都将违反《中华人民共和国著作权法》和其他相关法律法规以及国际公约的规定,杭州博日充分保留追究相应法律责任的权利。

联系方式

地址:杭州市高新(滨江)区滨安路1192号(310053)

总机:0571-87774567

免费送彩金的棋牌游戏-2019送彩金的娱乐平台-无需申请送18元彩金